HEPES - Wikipedia
HEPES (4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to ...
HEPES (1M) - Thermo Fisher Scientific
Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator. HEPES is a good buffering choice for ...
4- (2-Hydroxyethyl)-1-piperazine ethanesulfonic acid - PubChem
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 ℃.
HEPES Buffer - MilliporeSigma
HEPES is commonly used as a biological buffering agent in biochemical research that helps maintain a stable pH in aqueous solutions. It is effective as a buffer, especially in the pH range of 6.8 to 8.2.
HEPES - an overview | ScienceDirect Topics
HEPES is a buffer solution consisting of HEPES, MES, and sodium acetate dissolved in water, used to maintain a stable pH range from 5.0 to 7.5 in scientific experiments. AI generated definition based on: Methods in Cell Biology, 2021
Universal buffers for use in biochemistry and biophysical …
Individual curves for HEPES, MES, and NaAc show buffering range of specific weak acids. HEPES is a diprotic acid and has two relevant pKa’s. When combined into a universal buffer, a linear response to the addition of acid is observed across the entire tested range.
HEPES - the when, why, & how of using this pH buffer - YouTube
HEPES stands for N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid. People can’t agree on how to pronounce it, but many do agree that it is often a “Good” ...
HEPES - an overview | ScienceDirect Topics
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HEPES has been described as one of the best all-purpose buffers available for biological research.4 At most biological pHs the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture.
HEPES - an overview | ScienceDirect Topics
Regulation of oxygen sensing in peripheral arterial chemoreceptors. Sukhamay Lahiri, ... Donald G Buerk, in The International Journal of Biochemistry & Cell Biology, 2001. Many in vitro experiments on CB or CB glomus cells are done with HEPES medium at …
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